Abstract

The liver is unique in its ability to regenerate in response to injury. A number of evolutionary safeguards have allowed the liver to continue to perform its complex functions despite significant injury. Increased understanding of the regenerative process has significant benefit in the treatment of liver failure. Furthermore, understanding of liver regeneration may shed light on the development of cancer within the cirrhotic liver. This review will provide an overview of the models of study currently utilized in liver regeneration, the molecular basis of liver regeneration, and the role of liver progenitor cells in regeneration of the liver. Specific focus will be placed on clinical applications of current knowledge in liver regeneration including small for size liver transplant. Furthermore, cutting edge topics in liver regeneration including in vivo animal models for xenogeneic human hepatocyte expansion and the use of decellularized liver matrices as a three dimensional scaffold for liver repopulation will be proposed. Unfortunately, despite 50 years of intense study, many gaps remain in the scientific understanding of liver regeneration.

Regeneration of the liver can be more correctly defined as compensatory hyperplasia where in the remaining liver tissue expands to meet the metabolic needs of the organism. Unlike anatomic trueregeneration, the expanding liver does not regain its original gross anatomical structure¹ . It is also important to note the origin of cells utilized to replace the missing hepatocytes. Contrary to true regeneration, in the case of partial hepatectomy and some chemical liver injuries the liver mass is replaced by replication of existing hepatocytes without activation of progenitor cells. In other cases of chemical liver injury including galactosamine toxicity, activation and replication of progenitor cells does occur² .

Timing of Regeneration

Certain aspects of liver regeneration vary according to circadian rhythms. Matsuo and colleagues demonstrated that following partial hepatectomy in mice, the transition from G2 to mitosis occurred at the same time of day despite variability in the time of day the partial hepatectomy was performed³ . DNA synthesis, however, peaked at 36 hours after surgical intervention, irrespective of the light dark cycle employed. This data strongly supports that the transition from G2 to mitosis is controlled, at least in part, by circadian-dependent cell cycle-relate genes. Specifically, these genes modulate the expression of cyclin B1-Cdc2 kinase, an important regulator of mitosis. Matsuo further presentedwee1 as a candidate for the circadian regulator of hepatocyte division. At high levels, WEE1 phosphorylates Cdc2 kinase, disrupting the activity of the cyclin B1-Cdc2 kinase complex⁴ . Therefore, the progression of hepatocytes into mitosis is postponed until levels of WEE1 are low.

In contrast to the circadian rhythm regulated hepatocyte mitosis, DNA replication is independent of circadian rhythm but appears to be an intrinsic property of hepatocytes. There is species variation in peak DNA synthesis following partial hepatectomy with rat DNA synthesis peaking 12-16 hours earlier compared to mice. Weglarz and Sandgren demonstrated the timing of hepatocyte entry into DNA synthesis after partial hepatectomy is cell autonomous⁵ . They transplanted rat hepatocytes into the livers of mice after partial hepatectomy and found that the rat hepatocytes replicated earlier than mouse hepatocytes in the chimeric liver. This results defined DNA synthesis as cell autonomous and suggests that cytokines or growth factors may have a permissive but not an instructive role in hepatocyte progression to S phase.

Models for Liver Regeneration

A number of models have been proposed for the study of liver regeneration. The most completely studied model is that of liver regeneration following partial hepatectomy. A rodent model of two-thirds hepatectomy was first proposed by Higgins et al in 1931⁶ . The rodent liver is multilobar allowing for the removal of 3 of 5 liver lobes (⅔ of the liver mass). Within 5 — 7 days of surgical removal the remaining liver has regenerated to a size equivalent to the original mass. This model has remained a popular model of study as there is no injury to the residual liver. The resultant sequence of events can be clearly delineated without histologic evidence of damage to the residual liver tissue.

Zebrafish have been recognized as an exceedingly important model of developmental biology due to their prolific production of offspring and transparent embryos offering constant visualization and experimental manipulation. Furthermore, organogenesis occurs rapidly with presence of nearly all major organ systems by 2 days post fertilization; a mature liver is visualized under standard light microscope by 5 days post fertilization⁷ . Forward genetic screening, the technique of targeting embryonic mutants defective in a particular process, has allowed researchers to identify essential genes for various processes of hepatogenesis within this vertebrate model⁸ .

Chemical mediated hepatotoxic injury, including carbon tetrachloride, has also served as a common model of liver injury. The challenge of CCL4 mediated injury is that it triggers necrosis of lobular zones of the liver leading to acute inflammatory response. The inflammatory response is dominated by polymorphonuclear leukocytes and macrophages infiltrating the liver to remove necrotic hepatocytes. The intense inflammatory response is thought to affect both the onset and duration of liver regenerative response⁹ .

D-galactosamine is known to cause acute liver damage in animal models. The mechanism of D-galactosamine hepatotoxicity is not fully understood but D-galactosamine is believed to cause an intracellular deficiency of uridine metabolites leading to acute liver failure¹⁰. As illustrated in Figure 1, acute liver injury by D-galactosamine is associated with waste accumulation, systemic inflammation and impaired regeneration. These three problems are also seen in humans and often contribute to death after drug induced acute liver injury which makes the porcine model of D-galactosamine acute liver failure an appropriate large animal model for testing extracorporeal liver assist devices.

Acetaminophen intoxication is a common clinical cause of acute liver failure. Following overdose of acetaminophen the liver cannot perform the necessary breakdown steps of glucuronidation and sulphationand the P450 system takes over. Subsequently a toxic accumulation of N-acetyl-benzoquinoneimine occurs leading to the formation of radicals and Kupffer cell activation¹¹. The systemic manifestation of acetaminophen hepatotoxicity are believed to be mediated by proinflammatory cytokines and the innate immune system (see Figure 1). For example, mice with mutant Tol-like receptor 4 (TLR4) had significantly improved survival after acetaminophen overdose compared to normal wild-type mice. Furthermore, survival of wild-type mice was improved significantly both by depletion of Kupffer cells or pretreatment with a TLR 4 antagonist. Kupffer cells express high levels of TLR4¹². These studies show that reduction of TLR4 activity through clinical treatment is associated with mitigation of systemic inflammation and improved survival in a mouse model of acetaminophen-induced liver failure. They also show that the TLR4 activity of Kupffer cells is a main contributor to the systemic inflammatory response of acute liver failure, and that modulation of the TLR4 pathway by depletion of Kupffer cells or direct antagonism of TLR4 receptor leads to improved survival following acetaminophen-induced acute liver failure. Future studies should address whether improved survival is also the result of enhanced liver regeneration.

Genetically modified animals with inborn errors of metabolism have also been proposed to serve as models of liver regeneration. Most impressive may be theimmunodeficient, fumarylacetoacetate hydrolase (FAH)-deficient mouse model developed by Grompe et al. The livers of these triple knock-out mouse are capable of engraftment and significant repopulation with mature human hepatocytes following xenogeneic transplantation¹³ . Nyberg et al has produced (FAH)-deficient swine to upscale production of high quality human hepatocytes¹⁴. This in vivo environment allows primary hepatocytes to expand when incorporated into the three-dimensional liver architecture and exposed to the complex signaling pathways required for liver regeneration which cannot be achieved in an in vitro setting. Bissig and colleagues achieved up to a 95% repopulation of immunodeficient, FAHdeficient mouse liver with human hepatocytes following intrasplenic injection¹⁵ .

Molecular Basis of Liver Regeneration

The majority of evidence defining the molecular mechanisms associated with liver regeneration is derived from rodent models following partial hepatectomy. The numerous signaling pathways involved in liver regeneration are complex and interconnected. Genetic modifications resulting in defects in a single signaling pathway often result in delayed liver regeneration but do not completely prevent the regenerative process from occurring. Delays resulting from a single pathway disruption imply that the complex, often redundant, network of pathways is essential for liver regeneration to proceed in an optimal manner resulting in adequate hepatic mass⁹ . The pathways involved in liver regeneration include cytokines, growth factors, and metabolic networks¹⁶

Cytokine Signaling

Immediately following partial hepatectomy, greater than 100 immediate early genes are activated by transcription factors that are latent in the quiescent liver^{17, 18}. IL-6 is responsible for activating approximately 40% of these genes¹⁹. Activation of the immediate early genes results in a series of events including DNA synthesis, cell replication, and increase in cell size over several days. These immediate early genes also allow the liver maintain its essential metabolic functions during the process of liver regeneration9 .This process occurs in hepatocytes as well as the non-parenchymal liver cells, with hepatocytes replication occurring earlier than other cell types. Among hepatocytes there is an organized fashion by which DNA synthesis progresses, starting with the hepatocytes near the portal vein and proceeding to the cells adjacent to the central vein²⁰ .

The initiation of liver regeneration is driven by the innate immune system and cytokine release. TNF, NFΚB, and IL-6 are important mediators that result in activation of STAT3 in hepatocytes. Following partial hepatectomy, TNF binds to the TNF receptor 1 on nonparenchymal cells, primarily Kupffer cells. This leads to activation of NFΚB and production of IL-6²¹. IL-6 acts on hepatocytes via the IL-6 receptor, activating the signal transducer and activator of transcription 3 (STAT3) and extracellular signal-related kinase 1 and 2 (ERK1/2) pathways^22,23. Numerous studies have provided evidence relating this pathway to the initiation of liver regeneration. The additional of anti-TNF antibodies following partial hepatectomyinhibits IL-6 production and DNA replication in a rat model²⁴. The IL-6 knockout mouse leads to delays in liver regeneration²⁵. Hepatocyte proliferation and gene express can be corrected in this model with a single preoperative injection of IL-6. Interestingly, elevations in serum TNF following partial hepatectomy is not seen universally among all species studied. Tnfknockout mice proceed through a normal course of liver regeneration while Tnfr1 KO mice have multiple defects following partial hepatectomy suggesting that other ligands may bind to TNFr1^26,27 .

The innate immune system plays a role in the initiation of the cytokinecascade. Lipopolysaccharide (LPS), C3a, and C5a, all components of the innate immune system, bind to their respective receptors on Kupffer cells, triggering liver regeneration. Rats with restricted production of LPS have a delay in regeneration²⁸. Campbell and colleagues investigated the immune mediated signaling pathways involved in the initiation of liver regeneration²⁹. They evaluated mice lacking Tlr2 and Tlr2, the LPS co-receptor Cd14, and Myd88. MyD88 is an adaptor protein for the TLR family of proteins. They determined that Myd88 knockout mice had decreased levels of IL-6 and tnf mRNA following partial hepatectomy. Activation of STAT3 and STAT3-responsive genes were blocked as well. Interestingly, none of the knockout mice showed a delay in DNA replication. They concluded that the LPS receptor (TLR4), TLR2, and CD14 do not play a role in regulating cytokine production or DNA replication but that Myd88 –dependent pathways are involved in TNF and IL-6 production. The specific MyD88 associated receptors involved in the process have yet to be identified. A recent study by Vaquero and colleagues showed that TLR4 signaling contributes to IL-6 activation, but the Tlr4-independent component is sufficient for intact signaling downstream of IL-6³⁰. They demonstrated an attenuated increase in IL-6 after partial hepatectomy in mice with TLR4 signaling defects, supporting a role for LPS in triggering IL-6 activation. Regarding the role of complement in the initiation of liver regeneration, mice deficient in C3a and C5a have significant defects in regeneration following partial hepatectomy³¹. There is decreased activation of the cytokine pathway, diminishedelevation in TNF,and IL-6 levels and impaired NFΚB and STAT3 activity. This phenotype can be reversed by reconstitution of C3a and C5a.

IL-6 has multiple functions during liver regeneration including its role in the acute phase response, hepatoprotection, and mitogenesis²³. Many knockout mice studies have established that IL-6 is required for normal liver regeneration. However, as previously discussed, IL-6 is not the lone cytokine involved in the initiation of liver regeneration as the process is only delayed in the absence of IL-6. Following binding of IL-6 to its receptor on hepatocytes, the gp130 subunit is activated resulting in tyrosine kinase activity. This leads to activation and dimerization of STAT3 allowing for translocation to the nucleus where it activates transcription of target genes³². Stem cell factor (SCM) and oncostatin M (OCM) modulate and enhance the effects of IL-6 by activating STAT3^33,34. Studies in liver-specific stat3-null mice demonstrate a significant contribution of the IL-6 induced STAT3 pathway to immediate-early gene expression³⁵. This observed decline in immediate-early gene expression in the stat3-null mice was similar but not identical to the IL-6 knockout mice gene expression. This was the first study to provide evidence that STAT3 promotes cell cycle progression and proliferation in vivo, blurring the lines between growth factor and cytokine regulated pathways. STAT3 also functions as the main IL-6-mediated effector of hepatoprotection. STAT3 blocks apoptosis by increasing anti-caspase regulators and decreasing oxidative injury by increasing levels of the antioxidant REF1³⁶. IL-6 provides hepatoprotectionfrom Fas-mediated injury and apoptosis. IL-6−/− mice demonstrate alterations in the apoptotic pathways with reduction in anti-apoptotic factors³⁷.The IL-6 complex activation of gp130 also leads to activation of the MAPK signaling cascade. MAPK signaling is critical for cell proliferation. The stat3-null mice had normal activation of the MAPK pathway supporting the theory that not all effects of IL-6 on hepatocyte proliferation are mediated by STAT3.

Growth Factor-Mediated Pathways

Hepatocytes progress through the cell cycle in response to a collection of mitogenic growth factors. These growth factors override the G1 restriction point allowing hepatocytes to pass into the S phase. This passage involves Rb phosphorylation, increased expression of p107 and cyclins D, E, and A^38,39. In addition, cdk4/cyclinD and cdk2/cyclinE complexes are formed. The gp130 receptor subunit is involved with downstream production of the cyclins required for progression through the cell cycle.

The epidermal growth factor (EGF) receptor ligand family and hepatocyte growth factor (HGF) are important growth factors during liver regeneration⁴⁰. HGF is produced by stellate cells and act in a paracrine and endocrine fashion on hepatocytes. Pro-HGF is activated in the extracellular matrix by uPA⁴¹. HGF and c-met, the gene for the HGF receptor, are essential for liver regeneration⁴². HGF/c-met signaling results in activation of ERK1/2⁴³ . ERK1/2 has been shown to lead to hepatocyte proliferation in vitro and DNA replication in vivo. Other studies have suggested that the HGF/c-met pathway plays an important role in hepatoprotection by up regulating kinases involved in cell survival, specifically PI3K and AKT⁴⁴. The EGF receptor ligand family includes EGF, TGFα, heparin-binding EGF-like growth factor (HBEGF), and amphiregulin (AR). These various ligands have different but often overlapping functions. EGF is produced by Brunner’s gland in the duodenum⁴⁵ . TGFαis produced by hepatocytes in response to cell proliferation and functions in an autocrine fashion46. Increased levels ofTGFαresult in constitutive hepatocyte proliferation⁴⁷ . TGFα knockout models reveal normal liver regeneration following partial hepatectomy highlighting the overlapping roles of the multiple EGF receptor ligands⁴⁸ . HBEGF is expressed early in liver regeneration⁴⁹. A HBEGFknockout model leads to delayed liver regeneration with earlier expression of TGFα as a compensatory mechanism⁵⁰ . Beyond the compensatory mechanisms within the EGF receptor ligand family, there is some evidence to suggest the EGF receptor and HGF/c-met pathways may compensate for one another.

Auxiliary mitogens include TNF, IL-6, norepinephrine, Notch and jagged, VEGF, insulin, bile acids, serotonin, complement, leptin, estrogens, and FGF1 and 2⁵¹. Knockout models involving these mitogens will delay but not eliminate liver regeneration. Platelet-derived serotonin has been shown to mediate liver regeneration. The expression of 5-HT2A and 2B serotonin receptors increases in the liver following partial hepatectomy. In a series of experiments by Lesurtel et al, thrombocytopenia or impaired platelet activity results in failure to initiate hepatocyte proliferation in a mouse model⁵². Administration of a serotonin agonist in thrombocytopenic mice resulted in normal liver proliferation while administration of serotonin receptor antagonist led to inhibition of regeneration.

Overlap exists between the growth factor and cytokine mediated pathways. IL-6, HGF, and some EGF receptor ligands have been identified as promoting expression of ERK1/2. ERK1/2 activation results in DNA replication in vivo and proliferation in vitro^53,54 . Additionally, the gene encoding for insulin-like growth-factor-binding protein (IGFBP) may be activated both IL-6 and HGF. IGFBP is a mitogenic and hepato-protective proteinup regulated during regeneration^55,56 . IGFBP knockout models display impaired liver regeneration accompanied by delayed DNA synthesis, necrosis, and reduced expression of cyclins important for the S phase of the cell cycle. IGFBP is hepato-protective against Fasmediated injury and regulates apoptotic proteins MMP9 and TGFβ⁵⁷ .

Metabolic Pathways

Following partial hepatectomy, the metabolic demands on the liver during regeneration are immense. The liver must continue to support the organism during the regenerative process by providing an adequate systemic energy requirement while attempting to meet the energy demands needed for DNA replication and cell division. Amino acids regulate hepatocyte proliferation through modulation of cyclin D1 expression⁵⁸. Studies in rats indicate that administration of amino acids leads to hepatocyte replication, while protein restriction impairs regeneration^59,60. Translation is the control point that integrates nutrient levels with mitogenic signals; most proteins involved are downstream of mTOR (mammalian target of rapamycin)⁶¹. The mTOR complex may regulate regeneration by modulating cell size and proliferation based on energy demands⁶². Administration of rapamycin, an inhibitor of mTOR, inhibits DNA replication following partial hepatectomy⁶³ .

MicroRNAs

MicroRNAs (miRNAs) represent a relatively new class of gene expression regulators known to control cell proliferation in cancer. Work by Song, et al, has demonstrated the importance of miRNA in regulating hepatocyte proliferation during liver regeneration. Wild-type mice with inactivation of the DiGeorge syndrome critical region gene 8 (DGCR8), a critical region in the processing of miRNA,were found to exhibit delay in cell cycle progression from G1 to S phase. Post-mortem examination of livers from these mice demonstrated inhibition of miR-21 which is essential for DNA synthesis in hepatocytes following 2/3 hepatectomy; furthermore, miRNA-378 was repressed. miRNA-378 is responsible for inhibition of ornithine decarboxylase (Odc1) which in turn promotes DNA synthesis⁶⁴ . Additional research has demonstrated the influence of miRNA in embryonic development in zebrafish⁶⁵. Current efforts are aimed at identifying potential therapeutic applications of miRNA in various hepatic disease states⁶⁶ .

Termination of Liver Regeneration

Following partial hepatectomy, the liver rapidly regenerates to a size meeting the functional needs of the organism. The vast majority of research surrounding liver regeneration has focused on cytokine and growth factor mediated pathways involved in initiation and progression through the cell cycle. Yet, the mechanisms involved in termination of liver regeneration require critical review as they remain poorly understood. The termination process involves TGFβand feedback inhibition from the cytokine and growth factor pathways.

Suppressors of cytokine signaling (SOCS) are negative regulators of the cytokine signaling cascade. IL-6 signaling results in rapid up regulation of SOCS3. SOCS3 prevents phosphorylation of STAT3, leading to its down regulation⁶⁷. This results in a blockade of the IL-6 signal. This negative feedback loop explains why overexpression of IL-6 can lead to increased liver injury and impaired cell growth following partial hepatectomy⁶⁸. Without SOCS3, hepatocytes are hyperproliferative in response to growth factors in culture suggesting this protein is important in controlling the normal proliferative response in hepatocytes⁶⁹ .

TGFβ is an antiproliferative factor produced by stellate cells that is upregulated during liver regeneration in response to signaling from HGF and EGF^70,71. This increase in TGFβ is countered by a decrease in TGFβ receptors on hepatocytes during the first 48 hours following partial hepatectomy allowing for rapid proliferation⁷². In addition, norepinephrine may block the antiproliferative effects of TGFβearly during the regeneration process⁷³. Much controversy remains surrounding the specific mechanisms by which TGFβ modulates the regenerative process and overall data to support TGFβ as the primary stimulus for termination is lacking. ATGFβreceptor knockout model also revealed normal regulation unless activin, a member of theTGFβfamily, was also eliminated⁷⁴ .

Other theories surrounding the termination of liver regeneration focus on re-establishing the pre-partial hepatectomy extracellular matrix. TGFβhas a role in the assembly of the extracellular matrix and sinusoidal networks at the end of the regenerative process. As the extracellular matrix is re-established, it binds HGF, preventing activation and returning hepatocytes to their quiescent state⁷⁵. Additionally, TGFβ is increasingly bound to decorin in the extracellular matrix leading to a extracellular milieu similar to the pre-partial hepatectomy state. This return of HGF and TGFβ to baseline may lead to complete termination of liver regeneration⁵¹ .

Recent work completed by Wuestefeld et.al. identified the kinase MKK4 as a master regulator of liver regeneration⁷⁶. Silencing MKK4 resulted in an increased regenerative capacity of hepatocytes in mouse models of acute and chronic liver disease. MKK4 knockdown resulted in increased liver regeneration through faster hepatocyte entry into and progression through the cell cycle.

Liver Progenitor Cells

Liver progenitor cells are thought of as the second line of defense against liver injury, becoming active when mature hepatocytes are prevented from proliferating. Ongoing work in animal and human models of disease has helped to delineate the role of liver progenitor cells in the physiologic as well as the regenerating liver.

The first liver progenitor cells to be identified, termed oval cells, were described in 1956 by Farber et al⁷⁷. Oval cells, named for the appearance of their nucleus, are small bipotent cells with a high nuclear-cytoplasmic ratio which are capable of differentiation into both choangiocytes and hepatocytes⁷⁸. These cells have been shown to activate in animal studies in which native hepatocytes have been chemically blocked from proliferation in the setting of liver injury stimulating regeneration⁷⁹. Oval cells proliferate within the peri-portal region dependant on growth factors produced by stellate cells including HGF, FGF1, FGF2, and VEGF⁸⁰. The oval cell, capable of production of albumin and alpha-fetoprotein become basophilic hepatocytes within four to five days of activation. Eventually these cells can become mature hepatocytes⁸¹ .

Origin of Liver Progenitor Cells

The origin of liver progenitor cells has important clinical implications. If early progenitor cells can be identified and stimulated to proliferate within the injured liver, more rapid regeneration may occur. Initial work in bone marrow transplantation suggested liver progenitor cells were a continuous population with bone marrow stem cells. This hypothesis originated from studies demonstrating that female recipients of bone marrow transplants from male donors were found to have XY hepatocytes⁸². Subsequent studies utilizing a murine model of hereditary tyrosinemia demonstrated that the liver could be completely regenerated with bone marrow stem cells⁸³. Additional studies, however, have indicated that fusion was mechanism behind the regeneration of the liver following a bone marrow transplant⁸⁴ .

Liver progenitor cells have been noted to have both epithelial and mesenchymalmarkers ⁸⁵ . Furthermore, mesenchymal stem cells have been observed to convert to hepatocyte-like cells under appropriate conditions, thus researchers postulated that perhaps progenitor cells were derived from hepatocyte stellate cells. Subsequently Yang et al utilized the technique of fate mapping to demonstrate that stellate cells can go on to become oval cells following liver injury ⁸⁶. This theory suggested that stellate cells contribute to both fibrosis and regeneration by transition from epithelial to mesenchymal cell lines with subsequent reversion. Additional fate mapping by other researchers, however, has failed to demonstrate this phenomenon and this theory remains controversial ^{87, 88}.

Further study is needed to delineate the origin of progenitor cells. Current evidence is conflicting, however, many experts believe most liver progenitor cells are derived from in situ cells that are direct descendants of the fetal ductal plate ⁸⁵. Current efforts are directed at stimulating proliferation of stem cells or transplanting additional progenitor cells into the affected liver.

Liver Progenitor Cells Role in the Physiologic Liver

The role of progenitor cells in normal liver physiology is not completely understood, however, it is thought that progenitor cells have little involvement in day to day liver remodeling⁸⁹. Work by Suzuki et al demonstrates that patients with higher MELD scores demonstrate higher levels of progenitor cell activation over the normal liver⁹⁰. Regardless of etiology of disease (i.e. chronic alcoholism, viral hepatitis, or primary biliary cirrhosis), when native hepatocytes are blocked from proliferation, levels of progenitor cells increase^{89, 91}. Furthermore, work by Libberechtet al in patients with viral hepatitis has demonstrated that progenitor cells are surrounded by other liver progenitor cells of various differentiation suggesting ongoing maturation and repopulation from the progenitor cell lineage⁹² .

Acute liver failure patients also demonstrate an increased degree of progenitor cell proliferation over native liver. It has been postulated that at 50% loss of hepatocytes with decreased proliferation of mature hepatocytes triggers proliferation of the progenitor cell population⁹⁰. This further de-emphasizes the role of liver progenitor cells in the physiologic liver.

Clinical Implications

Among the transplant community, the increasing demand for high-quality transplantable livers far outweighs the donated organ supply. In an attempt to overcome this disparity, partial liver transplantation from a living donor is being performed with increasing frequency. The major limitation to this technique is providing the recipient with an adequately sized, functioning graft while maintaining a high safety profile for the donor operation. The success of partial liver transplant requires some degree of liver regeneration. Small-for-size-syndrome (SFSS) results from an inability of a small graft to regenerate and is the main limiting factor in expanding the role of partial liver transplant⁹³ . Small-for-size-syndrome is characterized clinically by prolonged cholestasis, intractable ascites, coagulopathy, and encephalopathy which manifest only 3 to 5 days following segmental liver transplant as the small partial graft is unable to meet the metabolic needs of the recipient⁹⁴. In the most severe cases, SFSS progresses to acidosis, hypoglycemia, septic shock, renal and pulmonary failure, and death without retransplantation. SFSS results from a failure of liver regeneration due to a combination of inadequate parenchymal volume, portal hyperperfusion, arterial hypoperfusion, and venous pathology⁹⁵. Significant parenchymal injury is not required for liver failure to develop in small grafts. A deficiency in cell cycle progression, via a p21 dependent block, causes liver failure in mice. This can be overcome by inhibition of p21⁹⁶. Earlier data suggested that a graft-to-recipient weight ratio of <0.8% or a liver volume <30% of standard estimated liver volume were risk factors for SFSS⁹⁷.

Recent data suggests that the exposure of small grafts to high portal blood flow impairs liver regeneration as sinusoidal congestion and hemorrhage have been identified in partial liver grafts in pigs⁹⁸. Portal hyperperfusion results from a smaller liver volume compared to the native liver along with pre-existing portal hypertension⁹³. As portal venous flow is relatively unregulated in the liver compared to arterial flow, the portal hyperperfusion leads to a compensatory decrease in arterial blood flow. This “arterial buffering response” further contributes to impaired liver regeneration⁹⁹. Successful partial liver transplantation relies on balancing the portal venous and hepatic artery flow and ensuring adequate hepatic venous drainage.

Various strategies are currently being investigated to overcome SFSS. Zhu and colleagues demonstrated improved serologic studies , decreased post-transplant hospital stay, and reduced infection related morbidity in patients receiving omega-3 fatty acid supplementation compared to patients receiving parenteral nutrition alone¹⁰⁰. Further evidence supporting the protective effect of fatty acid supplementation following partial hepatectomy is provided by Yan et.al¹⁰¹. Following 70% partial hepatectomy, rats administered polyunsaturated fatty acids showed enhanced expression of the LKB1-AMPK signaling pathway resulting in improved tight junction integrity and improved postoperative hepatic function. Another potential strategy for overcoming SFSS utilizes the secreted factors from mesenchymal stem cells to promote liver regeneration. Following partial hepatectomy, mice treated with mesenchymal stem cell conditioned culture media demonstrated improved regenerative capacity with upregulation of cytokines and growth factors involved in cell proliferation, angiogenesis, and anti-inflammatory responses¹⁰². Infusion of bone marrow mesenchymal stem cells also promotes proliferation of hepatocytes following extended hepatectomy¹⁰³ . Pentoxifylline (a TNFα inhibitor that enhances activation of the IL-6 signaling pathway) was evaluated in a recent study including 101 noncirrhotic patients undergoing major liver resection. Administration of the drug resulted in better volumetry in patients with small remnant livers¹⁰⁴. Finally, surgical interventions including splenic artery ligation, splenectomy, and creation of a portocaval shunt have been described in clinical and animal studies to decrease the portal hyperperfusion associated with SFSS¹⁰⁵.

As primary hepatocytes rapidly proliferate in vivo in response to the complex signaling pathways of liver regeneration and under the support of the liver’s three-dimensional architecture, efforts are underway to engineer liver scaffolds that can be transplanted and used for replacement of the liver function in patients with a failing liver. One such example is a recellularized liver graft using a decellularized liver matrix which has been successful using a rat model¹⁰⁶. Upscaling this technique to a larger, porcine animal model may allow for the development of recellularized liver matrices functioning as an auxiliary graft for transplantation in humans.

Unlike the liver regeneration that occurs following living donor liver transplantation, which follows the same patternas seen in rodent models after partial hepatectomy, in humans liver regeneration occurs more frequently after injury from an ischemic or toxic insult¹⁰⁷. With the majority of animal models utilizing partial hepatectomy as the inciting event for liver regeneration, it is difficult to correlate these results to the human liver’s regenerative strategy following damage by drug overdose, viral infection, or excessive alcohol consumption. More research will be required to better understand the pathways involved in liver regeneration following a toxic insult, where progenitor cells play a larger role. Additionally, cirrhosis, steatosis, and age can have detrimental effects on the liver’s ability to regenerate²³. Little evidence exists to explain how altered liver pathology affects the process of liver regeneration.

Concluding

Remarks Significant advances in biologic understanding and clinical applications of the regeneration of the liver have occurred in the past several decades. The field of liver regeneration has provided an appropriate model for the study of signal transduction and cell cycle in vivo. From a clinical perspective better understanding of the role of liver regeneration has allowed for more aggressive liver resections in the setting of malignancy and treatment strategies for cirrhosis. Multiple gaps exist in our current knowledge, the understanding of which would further support clinical treatment strategies including small for size transplantation and availability of high quality transplantable organs. Current research efforts including the use of animal models as in vivo vectors for high quality human hepatocytes represents a unique and important frontier in the field of liver regeneration.

Acknowledgments

All authors report no potential financial or personal conflicts of interest. All authors have read the journal’s policy on disclosure of potential conflicts of interest. No editorial support has been utilized in preparation of the manuscript.